Histone H3.1 antibody (AA 8-12)

Catalog No. ABIN362735

Product Details anti-Histone H3.1 Antibody

Target: Histone H3.1 (HIST1H3B)

Binding Specificity: AA 8-12

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone H3.1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: The antibody detects endogenous level of total Histone H3.1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific immunogen.

Immunogen: Peptide sequence around AA 8-12 (R-K-S-T-G) derived from Human Histone H3.1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Histone H3.1 antibody (ABIN362735)

Zhang, Zhou, Huang, Bi, Zhou: "Paeoniflorin reduced BLP-induced inflammatory response by inhibiting the NF-?B signal transduction in pathway THP-1 cells." in: Central-European journal of immunology / Polish Society for Immunology and eleven other Central-European immunological societies, Vol. 39, Issue 4, pp. 461-7, (2015) (PubMed).

Zhang, Zhao, Li, Li, Cai, Shen, Shi, Li, Liu, Zhang, Ye: "The nuclear import of oncoprotein hepatitis B X-interacting protein depends on interacting with c-Fos and phosphorylation of both proteins in breast cancer cells." in: The Journal of biological chemistry, Vol. 288, Issue 26, pp. 18961-74, (2013) (PubMed).

Liao, Dong, Wu, Yang, Wang, Guo, Chen, Xu, Wen: "Resolvin D1 attenuates inflammation in lipopolysaccharide-induced acute lung injury through a process involving the PPAR?/NF-?B pathway." in: Respiratory research, Vol. 13, pp. 110, (2013) (PubMed).

Chen, Evans, Evans: "FLASH knockdown sensitizes cells to Fas-mediated apoptosis via down-regulation of the anti-apoptotic proteins, MCL-1 and Cflip short." in: PLoS ONE, Vol. 7, Issue 3, pp. e32971, (2012) (PubMed).

Yu, Shan, Feng, Li, Zhu, Xie, Li, Li: "Triptolide ameliorates Crohn's colitis is associated with inhibition of TLRs/NF-?B signaling pathway." in: Fitoterapia, Vol. 82, Issue 4, pp. 709-15, (2011) (PubMed).

Ma, Zhang, Zhong, Pan, Chen, Wang, Andrew, Ji, Han: "Decreased expression of profilin 2 in oral squamous cell carcinoma and its clinicopathological implications." in: Oncology reports, Vol. 26, Issue 4, pp. 813-23, (2011) (PubMed).

Yao, Zhu, Zhao, Liu, Luo, Yin: "Arctigenin enhances chemosensitivity of cancer cells to cisplatin through inhibition of the STAT3 signaling pathway." in: Journal of cellular biochemistry, Vol. 112, Issue 10, pp. 2837-49, (2011) (PubMed).

Li, Zhang, Li, Wang, Wang, Han, Qian, Yang, Liu, Wei, Chen, Zhang, Zhang: "CHP2 activates the calcineurin/nuclear factor of activated T cells signaling pathway and enhances the oncogenic potential of HEK293 cells." in: The Journal of biological chemistry, Vol. 283, Issue 47, pp. 32660-8, (2008) (PubMed).

Target Details for Histone H3.1

Target: Histone H3.1 (HIST1H3B)

Alternative Name: Histone H3.1 (HIST1H3B Products)

Synonyms: H3 histone antibody, Hist1h2ai antibody, histone cluster 1, H2ai-like1 antibody, histone H3.1 antibody, Hht21p antibody, Hist1h2ail1 antibody, H3.1 antibody, HHT21 antibody

Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Molecular Weight: 17 kDa

NCBI Accession: NP_003521

UniProt: P68431