AKT 1/2/3 antibody (pTyr312, pTyr315, pTyr316)
-
- Target See all AKT 1/2/3 products
- AKT 1/2/3
-
Binding Specificity
- pTyr312, pTyr315, pTyr316
-
Reactivity
- Human, Mouse, Rat
-
Host
- Rabbit
-
Clonality
- Polyclonal
-
Conjugate
- This AKT 1/2/3 antibody is un-conjugated
-
Application
- Immunohistochemistry (IHC), Immunofluorescence (IF)
- Specificity
- The antibody detects endogenous level of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- Peptide sequence around phosphorylation site of pTyr315, 316, 312 (P-E-Y (p) -L-A) derived from Human AKT1, AKT2, AKT3. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Isotype
- IgG
-
-
- Application Notes
-
Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200 - Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
-
- Target
- AKT 1/2/3
- Alternative Name
- AKT1/AKT2/AKT3 (AKT 1/2/3 Products)
- Background
- General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. /General protein kinase capable of phosphorylating several known proteins. IGF-1 leads to the activation of AKT3, which may play a role in regulating cell survival. Capable of phosphorylating several known proteins. Truncated isoform 2/PKB gamma 1 without the second serine phosphorylation site could still be stimulated but to a lesser extent.
- Molecular Weight
- 60 kDa
- NCBI Accession
- NP_005456, NP_001617
- UniProt
- Q9Y243
-