HSP27 antibody (pSer82)
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- Target See all HSP27 (HSPB1) Antibodies
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
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Binding Specificity
- pSer82
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This HSP27 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- The antibody detects endogenous level of HSP27 only when phosphorylated at serine 82.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- Peptide sequence around phosphorylation site of pSer82 (Q-L-S (p) -S-G) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Isotype
- IgG
- Top Product
- Discover our top product HSPB1 Primary Antibody
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- Application Notes
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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- Target
- HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))
- Alternative Name
- HSP27 (HSPB1 Products)
- Synonyms
- CMT2F antibody, HMN2B antibody, HS.76067 antibody, HSP27 antibody, HSP28 antibody, Hsp25 antibody, SRP27 antibody, 27kDa antibody, Hsp27 antibody, cb153 antibody, cb660 antibody, hsp1 antibody, hsp25 antibody, hsp27 antibody, id:ibd2821 antibody, sb:cb660 antibody, zgc:103437 antibody, HSPB1 antibody, cmt2f antibody, hs.76067 antibody, hsp28 antibody, heat shock protein family B (small) member 1 antibody, heat shock protein 1 antibody, heat shock protein, alpha-crystallin-related, 1 antibody, heat shock protein family B (small) member 1 L homeolog antibody, HSPB1 antibody, Hspb1 antibody, hspb1 antibody, hspb1.L antibody
- Background
- Involved in stress resistance and actin organization.
- Molecular Weight
- 27 kDa
- NCBI Accession
- NP_001531
- UniProt
- P04792
- Pathways
- MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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