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MAP2K6 antibody (pSer207)
MAP2K6
Reactivity: Human, Rat
WB, IHC, IF
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-MAP2K6 Antibody
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Target
See all MAP2K6 Antibodies
MAP2K6
(Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
Binding Specificity
All epitopes for MAP2K6 antibodies
pSer207
Reactivity
All reactivities for MAP2K6 antibodies
Human, Rat
Host
All hosts for MAP2K6 antibodies
Rabbit
Clonality
All clonalities for MAP2K6 antibodies
Polyclonal
Conjugate
All conjugates for MAP2K6 antibodies
This MAP2K6 antibody is un-conjugated
Application
All applications for MAP2K6 antibodies
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of MKK6 only when phosphorylated at serine207.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer207 (V-D-S (p) -V-A) derived from Human MKK6. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype
IgG
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target Details for MAP2K6
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Target
MAP2K6
(Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
Alternative Name
MKK6 (MAP2K6 Products )
Synonyms
MAPKK6 antibody, MEK6 antibody, MKK6 antibody, PRKMK6 antibody, SAPKK-3 antibody, SAPKK3 antibody, Mkk6 antibody, Prkmk6 antibody, MKK3 antibody, map2k3 antibody, zMKK3 antibody, map2k6 antibody, mitogen-activated protein kinase kinase 6 antibody, mitogen-activated protein kinase kinase 6 L homeolog antibody, MAP2K6 antibody, Map2k6 antibody, map2k6 antibody, map2k6.L antibody
Background
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
Molecular Weight
41 kDa
NCBI Accession
NP_002749
UniProt
P52564
Pathways
MAPK Signaling , TLR Signaling , Activation of Innate immune Response , Regulation of Muscle Cell Differentiation , Toll-Like Receptors Cascades
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