The antibody detects endogenous level of Myosin Light Chain 2 only when phosphorylated at serine 18.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer18 (A-T-S (p) -N-V) derived from Human Myosin Light Chain 2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion