tau antibody (pSer396)
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- Target See all tau Antibodies
- tau (tau Protein)
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Binding Specificity
- pSer396
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This tau antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC)
- Specificity
- The antibody detects endogenous level of Tau only when phosphorylated at serine396.
- Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
- Immunogen
- Peptide sequence around phosphorylation site of pSer396 (Y-K-S (p) -P-V) derived from Human Tau. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
- Isotype
- IgG
- Top Product
- Discover our top product tau Primary Antibody
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- Application Notes
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Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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Synaptic released zinc promotes tau hyperphosphorylation by inhibition of protein phosphatase 2A (PP2A)." in: The Journal of biological chemistry, Vol. 287, Issue 14, pp. 11174-82, (2012) (PubMed).
: "Neuroglobin attenuates Alzheimer-like tau hyperphosphorylation by activating Akt signaling." in: Journal of neurochemistry, Vol. 120, Issue 1, pp. 157-64, (2011) (PubMed).
: "Protective effects of lithium treatment for spatial memory deficits induced by tau hyperphosphorylation in splenectomized rats." in: Clinical and experimental pharmacology & physiology, Vol. 37, Issue 10, pp. 1010-5, (2010) (PubMed).
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Synaptic released zinc promotes tau hyperphosphorylation by inhibition of protein phosphatase 2A (PP2A)." in: The Journal of biological chemistry, Vol. 287, Issue 14, pp. 11174-82, (2012) (PubMed).
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- Target
- tau (tau Protein)
- Alternative Name
- Tau (tau Products)
- Synonyms
- DDPAC antibody, FTDP-17 antibody, MAPTL antibody, MSTD antibody, MTBT1 antibody, MTBT2 antibody, PPND antibody, TAU antibody, tau antibody, PHF-tau antibody, microtubule associated protein tau antibody, MAPT antibody
- Background
- Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
- Molecular Weight
- 78 kDa
- UniProt
- P10636
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