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GATA1 antibody (pSer310)
GATA1
Reactivity: Human, Mouse, Rat
WB, IF
Host: Rabbit
Polyclonal
unconjugated
Product Details anti-GATA1 Antibody
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Target
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GATA1
(GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
Binding Specificity
All epitopes for GATA1 antibodies
pSer310
Reactivity
All reactivities for GATA1 antibodies
Human, Mouse, Rat
Host
All hosts for GATA1 antibodies
Rabbit
Clonality
All clonalities for GATA1 antibodies
Polyclonal
Conjugate
All conjugates for GATA1 antibodies
This GATA1 antibody is un-conjugated
Application
All applications for GATA1 antibodies
Western Blotting (WB), Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of GATA1 only when phosphorylated at serine 310.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer310 (K-A-S (p) -G-K) derived from Human GATA1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype
IgG
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Alternatives
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Application Details
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Application Notes
Western blotting: 1:500-1:1000 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target Details for GATA1
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Target
GATA1
(GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
Alternative Name
GATA1 (GATA1 Products )
Synonyms
ERYF1 antibody, GATA-1 antibody, GF-1 antibody, GF1 antibody, NF-E1 antibody, NFE1 antibody, XLANP antibody, XLTDA antibody, XLTT antibody, Gata-1 antibody, Gf-1 antibody, eryf1 antibody, Eryf1 antibody, GATA1 antibody, gata-1 antibody, Gata1 antibody, si:dkeyp-77f7.5 antibody, gata1 antibody, gata1a-A antibody, xGATA-1 antibody, xgata1 antibody, XGATA-1B antibody, gata1b-A antibody, gta1 antibody, zg1 antibody, NF-E1a antibody, GATA binding protein 1 antibody, GATA binding protein 1 (globin transcription factor 1) antibody, amidase antibody, aspartyl/glutamyl-tRNA amidotransferase subunit A antibody, putative amidase antibody, GatA1 antibody, glutamyl-tRNA amidotransferase subunit A antibody, hematopietic transcription factor GATA-1 antibody, GATA binding protein 1b antibody, GATA binding protein 1 L homeolog antibody, GATA binding protein 1 S homeolog antibody, GATA binding protein 1a antibody, erythroid transcription factor antibody, GATA1 antibody, Gata1 antibody, gatA1 antibody, gata-1 antibody, gata1b antibody, gata1.L antibody, gata1.S antibody, gata1a antibody, LOC108348091 antibody
Background
GATA1 encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.
Molecular Weight
43 kDa
NCBI Accession
NP_002040
UniProt
P15976
Pathways
Cellular Response to Molecule of Bacterial Origin
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