Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Specificity
The antibody detects endogenous level of GSK3a only when phosphorylated at serine 21and does not detect GSK-3b when phosphorylated at Ser9.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pSer21 (T-S-S (p) -F-A) derived from Human GSK3a. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
GSK3a
Reactivity: Human
WB, ELISA, IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100 Immunofluorescence: 1:100-1:200
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Liu, Huang, Zeng, Chen, Huang, Guo, Liu, Xu, Mo, Li: "Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway." in: Journal of cancer research and clinical oncology, Vol. 137, Issue 11, pp. 1629-40, (2011) (PubMed).