HSPB8 antibody

Catalog No. ABIN361852

Product Details anti-HSPB8 Antibody

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSPB8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Detects ~22 kDa. Does not cross-react with HSP27 or alpha-crystallin.

Cross-Reactivity: Human, Mouse, Rat

Purification: Peptide Affinity Purified

Immunogen: Human HSP22

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:100)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN361851 was sufficient for detection of HSP22 in 10 μg of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-HSPB8 antibody (ABIN361852)

Webster, Darling, Sanders, Blazier, Vidal-Aguiar, Beaulieu-Abdelahad, Plemmons, Hill, Uversky, Bickford, Dickey, Blair: "Hsp22 with an N-Terminal Domain Truncation Mediates a Reduction in Tau Protein Levels." in: International journal of molecular sciences, Vol. 21, Issue 15, (2020) (PubMed).

Target Details for HSPB8

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Alternative Name: HSP22 (HSPB8 Products)

Background: HSP22 (HSPB8) is a 196-amino acid protein that is a member of the small heat shock protein super-family and the human protein is most closely related to HSP27. Similar to most other sHSPs, HSP22 is predominately transcribed in skeletal muscle and heart, as well as the placenta (1). HSP22 is a monomeric protein which interacts with HSPB1. It displays temperature-dependent chaperone activity. In a two hybrid screen, HSPB8 interacted preferentially with a triple aspartate form of HSP27 which mimics HSP27 phosphorylated at Ser15, Ser78, and Ser82, as compared to wild-type HSP27 (2). HSPB8 has two binding domains (N and C Terminal) that are specific for different binding partners, and has the ability to bind itself and other sHSPs (3). The chaperone-like activity is of great importance to the function of HSP22 in various processes including proliferation, apoptosis and macro autophagy (4). Mutations in the HSPB8 gene are associated with the inherited peripheral neuropathies, autosomal dominant distal hereditary motor neuropathy type IIA (dSMA) and axonal Charcot-Marie-Tooth disease type 2L (CMT2L) (5).

Gene ID: 26353

NCBI Accession: NP_055180

UniProt: Q9UJY1