Calreticulin antibody

Catalog No. ABIN361835

Product Details anti-Calreticulin Antibody

Target: Calreticulin (CALR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calreticulin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~63 kDa.

Cross-Reactivity: Chicken, Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep

Purification: Peptide Affinity Purified

Immunogen: Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH

Application Details

Application Notes:

• WB (1:1000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN361834 was sufficient for detection of Calreticulin in 20 μg of HeLa cell lysate by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-Calreticulin antibody (ABIN361835)

Schultheis, Smith, Kiosses, Kraynyak, Wong, Oh, Broderick: "Delineating the Cellular Mechanisms Associated with Skin Electroporation." in: Human gene therapy methods, Vol. 29, Issue 4, pp. 177-188, (2019) (PubMed).

Koide, Kondo, Odkhuu, Ulziisaikhan, Ukaji, Yokochi, Umezawa: "Inhibition of receptor activator of nuclear factor-κB ligand- or lipopolysaccharide-induced osteoclast formation by conophylline through downregulation of CREB." in: Immunology letters, Vol. 161, Issue 1, pp. 31-7, (2014) (PubMed).

Kobayashi, Hoshinaga, Miura, Tokuda, Shigeoka, Murai, Horio: "Ascorbic acid deficiency decreases hepatic cytochrome P-450, especially CYP2B1/2B2, and simultaneously induces heme oxygenase-1 gene expression in scurvy-prone ODS rats." in: Bioscience, biotechnology, and biochemistry, Vol. 78, Issue 6, pp. 1060-6, (2014) (PubMed).

Frenz, Graalmann, Detje, Döring, Grabski, Scheu, Kalinke et al.: "Independent of plasmacytoid dendritic cell (pDC) infection, pDC triggered by virus-infected cells mount enhanced type I IFN responses of different composition as opposed to pDC stimulated with free ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 193, Issue 5, pp. 2496-503, (2014) (PubMed).

Kluge, Mack, Iyer, Pujol, Heigele, Learn, Usmani, Sauter, Joas, Hotter, Bibollet-Ruche, Plenderleith, Peeters, Geyer, Sharp, Fackler, Hahn, Kirchhoff: "Nef proteins of epidemic HIV-1 group O strains antagonize human tetherin." in: Cell host & microbe, Vol. 16, Issue 5, pp. 639-50, (2014) (PubMed).

Tongu, Harashima, Yamada, Harada, Harada: "Immunogenic chemotherapy with cyclophosphamide and doxorubicin against established murine carcinoma." in: Cancer immunology, immunotherapy : CII, Vol. 59, Issue 5, pp. 769-77, (2010) (PubMed).

Target Details for Calreticulin

Target: Calreticulin (CALR)

Alternative Name: Calreticulin (CALR Products)

Synonyms: CRT antibody, RO antibody, SSA antibody, cC1qR antibody, crc antibody, crt antibody, Calregulin antibody, CG9429 antibody, Clr antibody, Dmel\\CG9429 antibody, anon-EST:Liang-2.11 antibody, anon-EST:Liang-2.17 antibody, anon-EST:fe3D7 antibody, clone 2.11 antibody, clone 2.17 antibody, eth antibody, potp antibody, cc1qr antibody, ssa antibody, NV19007 antibody, CRP55 antibody, ERp60 antibody, HACBP antibody, CHOT1 antibody, CHT1 antibody, CT1 antibody, AA589632 antibody, CRTR antibody, Creat antibody, CCDS1 antibody, calrl2 antibody, fc66d10 antibody, wu:fc66d10 antibody, wu:fj50c03 antibody, zgc:66153 antibody, calreticulin antibody, calreticulin L homeolog antibody, Calreticulin antibody, putative calreticulin antibody, solute carrier family 6 member 8 antibody, solute carrier family 6 (neurotransmitter transporter, creatine), member 8 antibody, CALR antibody, calr.L antibody, Crt antibody, crt antibody, CRT antibody, Calr antibody, calr antibody, LMJF_31_2600 antibody, Crc antibody, Slc6a8 antibody, SLC6A8 antibody, LOC8269812 antibody

Background: Calreticulin is a multifunctional, highly conserved Ca2+ -binding protein that is localized to the endoplasmic reticulum (ER), but has also been detected in the nucleus and nuclear envelop. Like many other ER proteins, it has the conserved ER retention KDEL (Lys-Asp-Glu-Leu) sequence at its C-terminus (1-3). CRT's three domains include a 180 residue N-terminal domain, a proline-rich P-domain (residues 189-288) that binds Ca2+ with high affinity and shares homology with calnexin (CNX) and calmegin, and a 110 residue C-terminal domain that binds Ca2+ with low affinity but high capacity (1,3). Recent studies suggest that this soluble ER protein has a multifunctional role. It appears to be involved in calcium storage and regulation as well as having a molecular chaperone activity. It has been shown to interact with the cytoskeleton and to be involved in the regulation of gene expression. Calreticulin may also play a role in cellular proliferation including its apparent activity in the proliferation of certain viruses within mammalian host cells (4, 5), and it has also been shown to be induced in response to various types of cell stress including amino acid deprivation (6). Close interconnections among protein synthesis, gene expression and calcium signaling have been observed by many researchers in recent years. Calreticulin might be centrally located and therefore it crucially participates in the coordination of many functions by the cell (4, 5). Studies also suggest its involvement in a few diseases such as systemic lupus erythematosus, rheumatoid arthritis, celiac disease, complete congenital heart block, and halothane hepatitis (1).

Gene ID: 811

NCBI Accession: NP_004334

UniProt: P27797

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response