HSP90 antibody

Catalog No. ABIN361823

Product Details anti-HSP90 Antibody

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP90 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~90 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein A Purified

Immunogen: Full length protein HSP90

Application Details

Application Notes:

• WB (1:500)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:500 dilution of ABIN361822 was sufficient for detection of 0.2 mg of purified HSP90 by ECL immunoblot analysis.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-HSP90 antibody (ABIN361823)

Bacci, Aiello, Ripoli, Loria, Pugliese, Pierconti, Rotili, Strigari, Pinto, Bassi, Mai, Grassi, Pontecorvi, Falcioni, Farsetti, Nanni: "H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer." in: International journal of molecular sciences, Vol. 20, Issue 16, (2019) (PubMed).

Re, Colussi, Nanni, Aiello, Bacci, Grassi, Pontecorvi, Farsetti: "Nucleoporin 153 regulates estrogen-dependent nuclear translocation of endothelial nitric oxide synthase and estrogen receptor beta in prostate cancer." in: Oncotarget, Vol. 9, Issue 46, pp. 27985-27997, (2018) (PubMed).

Zhang, Pruitt, Tran, Du Bois, Zhang, Patel, Hoover, Simpson, Simmons, Gary, Snapper, Casellas, Mock: "B cell-specific deficiencies in mTOR limit humoral immune responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 4, pp. 1692-703, (2013) (PubMed).

Verheyen, Peeraer, Nuydens, Dhondt, Poesen, Pintelon, Daniels, Timmermans, Meert, Carmeliet, Lambrechts: "Systemic anti-vascular endothelial growth factor therapies induce a painful sensory neuropathy." in: Brain : a journal of neurology, Vol. 135, Issue Pt 9, pp. 2629-41, (2012) (PubMed).

Wagatsuma, Shiozuka, Kotake, Takayuki, Yusuke, Mabuchi, Matsuda, Yamada: "Pharmacological inhibition of HSP90 activity negatively modulates myogenic differentiation and cell survival in C2C12 cells." in: Molecular and cellular biochemistry, Vol. 358, Issue 1-2, pp. 265-80, (2011) (PubMed).

Target Details for HSP90

Target: HSP90 (Heat Shock Protein 90 (HSP90))

Alternative Name: HSP90 (HSP90 Products)

Synonyms: EL52 antibody, HSP86 antibody, HSP89A antibody, HSP90A antibody, HSP90N antibody, HSPC1 antibody, HSPCA antibody, HSPCAL1 antibody, HSPCAL4 antibody, HSPN antibody, Hsp89 antibody, Hsp90 antibody, LAP2 antibody, git10 antibody, swo1 antibody, HSP90 antibody, htpG antibody, SCBAC25F8.08 antibody, 23.m06066 antibody, 17.m07646 antibody, HSP90-1 antibody, 143198_at antibody, 83 antibody, 83K HSP antibody, DMHSP82 antibody, E(sev)3A antibody, E(sina)2 antibody, HSP82 antibody, HSP83 antibody, ORF1 antibody, Su(Raf)3A antibody, anon-EST:Liang-2.53 antibody, anon-WO0068693 antibody, anon-WO0140519.209 antibody, clone 2.53 antibody, en(lz)3C/4C antibody, hsp84 antibody, l(3)j5C2 antibody, ms(3)08445 antibody, stc antibody, DmelCG1242 antibody, CG1242 antibody, Hsp86 antibody, Hspca antibody, 86kDa antibody, 89kDa antibody, AL024080 antibody, AL024147 antibody, Hsp86-1 antibody, hsp4 antibody, hsp86 antibody, hsp89 antibody, hsp90 antibody, hsp90a antibody, hspc1 antibody, hspca antibody, hspn antibody, lap2 antibody, Hsp90alpha antibody, heat shock protein 90 alpha family class A member 1 antibody, heat shock protein Hsp90 antibody, Hsp90 chaperone antibody, Heat shock protein 90 antibody, heat shock protein 90 antibody, chaperone protein HtpG antibody, molecular chaperone HtpG antibody, Heat Shock Protein 90 antibody, uncharacterized LOC100384473 antibody, Heat shock protein 83 antibody, heat shock protein 90, alpha (cytosolic), class A member 1 antibody, heat shock protein 90 alpha family class B member 1 antibody, heat shock protein 90kDa alpha family class A member 1 L homeolog antibody, heat shock protein HSP 90-alpha antibody, HSP90AA1 antibody, hsp90 antibody, HSP90 antibody, daf-21 antibody, htpG antibody, SCO7516 antibody, MAP_RS10510 antibody, TP04_0646 antibody, TP01_0934 antibody, APH_RS03525 antibody, GbCGDNIH1_0315 antibody, MAV_2118 antibody, HSP90C antibody, BBOV_IV008400 antibody, BBOV_III007380 antibody, ACICU_00312 antibody, ECL_01244 antibody, YE105_C1172 antibody, pco153543(105) antibody, Hsp83 antibody, Hsp90aa1 antibody, HSP90AB1 antibody, hsp90aa1.1.L antibody, LOC108698781 antibody

Background: HSP90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, HSP90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, HSP90 is one of the most highly expressed proteins in unstressed cells (1-2 % of cytosolic protein). It carries out a number of housekeeping functions - including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the HSP90-regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with HSP90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5. When bound to ATP, HSP90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, HSP90-interacting proteins have been shown to co-precipitate with HSP90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in HSP90 expression or HSP90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit HSP90 function (7). Looking for more information on HSP90? Visit our new HSP90 Scientific Resource Guide at http://www.HSP90.ca.

Gene ID: 3326

NCBI Accession: NP_031381

UniProt: P08238

Pathways: M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals