PDIA3 antibody

Catalog No. ABIN361815

Product Details anti-PDIA3 Antibody

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PDIA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Specificity: Detects ~57 kDa.

Cross-Reactivity: Cow, Dog, Guinea Pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat

Purification: Protein G Purified

Immunogen: Human recombinant ERp57 (Grp58)

Clone: Map-ERp57

Isotype: IgG1

Application Details

Application Notes:

• WB (1:2000)
• IHC (1:100)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml of ABIN361814 was sufficient for detection of ERp57 in 10 μg of heat shock Heal Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-PDIA3 antibody (ABIN361815)

Ortiz-Sandoval, Hughes, Dacks, Simmen: "Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins." in: Cellular logistics, Vol. 4, Issue 4, pp. e986399, (2015) (PubMed).

Lynes, Raturi, Shenkman, Ortiz Sandoval, Yap, Wu, Janowicz, Myhill, Benson, Campbell, Berthiaume, Lederkremer, Simmen: "Palmitoylation is the switch that assigns calnexin to quality control or ER Ca2+ signaling." in: Journal of cell science, Vol. 126, Issue Pt 17, pp. 3893-903, (2013) (PubMed).

Target Details for PDIA3

Target: PDIA3 (Protein Disulfide Isomerase Family A, Member 3 (PDIA3))

Alternative Name: ERp57 (PDIA3 Products)

Synonyms: ER60 antibody, ERp57 antibody, ERp60 antibody, ERp61 antibody, GRP57 antibody, GRP58 antibody, HsT17083 antibody, P58 antibody, PI-PLC antibody, grp-58 antibody, 58kDa antibody, Erp antibody, Grp58 antibody, PDI antibody, PDI-Q2 antibody, PLC[a] antibody, Plca antibody, 1 antibody, 25D3-membrane-associated antibody, sb:cb825 antibody, erp57 antibody, grp58 antibody, pdia3 antibody, protein disulfide isomerase family A member 3 antibody, protein disulfide-isomerase A3 antibody, protein disulfide isomerase associated 3 antibody, protein disulfide isomerase family A, member 3 antibody, protein disulfide isomerase family A member 3 S homeolog antibody, PDIA3 antibody, Tsp_10062 antibody, Pdia3 antibody, pdia3 antibody, pdia3.S antibody

Background: ERp57, also known as Glucose Regulated Protein 58 (Grp58), Hormone-Induced Protein-70 (HIP-70) and microsomal Carnitine Palmitoyltransferase, is a member of the protein disulfide isomerase family, containing two canonical CXHC tetrapeptide active site motifs (1-5). It has quite a few diverse roles. It functions as an accessory oxidoreductase involved in disulfide bond formation. In the ER, ERp57 interacts with membrane bound calnexin and soluble calreticulin (lectin chaperones) via their praline rich P-domain arms. Lectin chaperones bind nascent non-native glycoproteins, and position ERp57 to act upon the immature or misfolded glycoproteins that possess mono-glycosylated side chains. ERp57 deletion impairs posttranslational phases of influenza hema-glutinin folding, and causes accelerated release of MHC-I molecules, resulting in the coupling of sub-optimal peptides and reduced expression and stability on the cell surface (6). ERp57 also contains two thioredoxin active-site sequences, CGHC and an estrogen-binding domain. ERp57 is induced by both estrogen and leuteinizing-hormone-releasing hormone in the hippocampus (7).

Gene ID: 2923

NCBI Accession: NP_005304

UniProt: P30101

Pathways: Maintenance of Protein Location, Protein targeting to Nucleus, Cell RedoxHomeostasis