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Hsc70 antibody

HSPA8 Reactivity: Human WB, IHC, ELISA, IP, ICC, IF, PLA, Bind, AA Host: Mouse Monoclonal 1F2-H5 unconjugated
Catalog No. ABIN361801
  • Target See all Hsc70 (HSPA8) Antibodies
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    Reactivity
    • 81
    • 48
    • 44
    • 21
    • 17
    • 14
    • 13
    • 11
    • 11
    • 11
    • 10
    • 10
    • 5
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 70
    • 27
    • 7
    • 2
    Mouse
    Clonality
    • 70
    • 35
    Monoclonal
    Conjugate
    • 58
    • 19
    • 11
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Hsc70 antibody is un-conjugated
    Application
    • 92
    • 51
    • 39
    • 28
    • 28
    • 27
    • 15
    • 14
    • 10
    • 10
    • 10
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Proximity Ligation Assay (PLA), Binding Studies (Bind), Antibody Array (AA)
    Specificity
    Detects ~73 kDa. Does not cross react with HSP70.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Full length human HSC70
    Clone
    1F2-H5
    Isotype
    IgG2a kappa
    Top Product
    Discover our top product HSPA8 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN361800 was sufficient for detection of HSC70 in 10 μg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Katoh, Kubota, Kita, Nakatsu, Aoki, Mori, Maenaka, Takeda, Kidokoro: "Heat shock protein 70 regulates degradation of the mumps virus phosphoprotein via the ubiquitin-proteasome pathway." in: Journal of virology, Vol. 89, Issue 6, pp. 3188-99, (2015) (PubMed).

    Shimomura, Imai, Nashida: "Characterization of cysteine string protein in rat parotid acinar cells." in: Archives of biochemistry and biophysics, Vol. 538, Issue 1, pp. 1-5, (2013) (PubMed).

    Baaklini, Wong, Hantouche, Patel, Shrier, Young: "The DNAJA2 substrate release mechanism is essential for chaperone-mediated folding." in: The Journal of biological chemistry, Vol. 287, Issue 50, pp. 41939-54, (2012) (PubMed).

    Kawano, Oke, Nomura, Fujita, Ohira, Nakai, Ohira: "Responses of HSC70 expression in diencephalon to iron deficiency anemia in rats." in: The journal of physiological sciences : JPS, Vol. 61, Issue 6, pp. 445-56, (2011) (PubMed).

  • Target
    Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))
    Alternative Name
    HSC70 (HSP73) (HSPA8 Products)
    Synonyms
    hsc54 antibody, hsc70 antibody, hsc71 antibody, hsp71 antibody, hsp73 antibody, hspa10 antibody, lap1 antibody, nip71 antibody, HSC54 antibody, HSC70 antibody, HSC71 antibody, HSP71 antibody, HSP73 antibody, HSPA10 antibody, LAP1 antibody, NIP71 antibody, Hsc70 antibody, 2410008N15Rik antibody, Hsc71 antibody, Hsc73 antibody, Hsp73 antibody, Hspa10 antibody, wu:fb01g06 antibody, wu:fi48b06 antibody, heat shock protein family A (Hsp70) member 8 L homeolog antibody, heat shock protein family A (Hsp70) member 8 antibody, heat shock 70kDa protein 8 antibody, heat shock protein 8 antibody, hspa8.L antibody, HSPA8 antibody, Hspa8 antibody, hspa8 antibody
    Background
    HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed, HSP 70 is highly related to HSC70 (>90 % sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (6).
    Gene ID
    3312
    NCBI Accession
    NP_006588
    UniProt
    P11142
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