CRYAA antibody

Catalog No. ABIN361799

Product Details anti-CRYAA Antibody

Target: CRYAA (Crystallin, alpha A (CRYAA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CRYAA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~20 kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Native Alpha Crystallin

Clone: 1H3-B8

Isotype: IgG1

Application Details

Application Notes:

• WB (1:2000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

0.5 μg/ml was sufficient for detection of 100 ng purified alphaA crystalline by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for CRYAA

Target: CRYAA (Crystallin, alpha A (CRYAA))

Alternative Name: Alpha A Crystallin (CRYAA Products)

Synonyms: CRYA1 antibody, CTRCT9 antibody, HSPB4 antibody, AI323437 antibody, Acry-1 antibody, Crya-1 antibody, Crya1 antibody, DAcry-1 antibody, lop18 antibody, alpha-a-crystallin antibody, cryaa-A antibody, CRYAA antibody, wu:fc06h04 antibody, zgc:92036 antibody, HspB4 antibody, crystallin alpha A antibody, crystallin, alpha A antibody, alphaA-crystallin antibody, alpha-crystallin A chain-like antibody, crystallin alpha A S homeolog antibody, alpha-crystallin A chain antibody, CRYAA antibody, Cryaa antibody, CpipJ_CPIJ005646 antibody, cryaa antibody, LOC100384978 antibody, cryaa.S antibody, LOC458589 antibody, LOC102724652 antibody

Background: The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its' phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8).

Gene ID: 1409

NCBI Accession: NP_000385

UniProt: P02489

Pathways: M Phase