DNA Damage antibody

Catalog No. ABIN361744

Product Details

Target: DNA Damage

Reactivity: All Species

Host: Mouse

Clonality: Monoclonal

Application: Dot Blot (DB), ELISA, Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).

Purification: Protein G Purified

Immunogen: 8-hydroxy-guanosine-BSA and -casein conjugates

Clone: 15A3

Isotype: IgG2b

Application Details

Application Notes:

• IHC (1:1000)
• optimal dilutions for assays should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Wu, Scarlata, OFlaherty: "Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 2, (2020) (PubMed).

Liu, OFlaherty: "In vivo oxidative stress alters thiol redox status of peroxiredoxin 1 and 6 and impairs rat sperm quality." in: Asian journal of andrology, Vol. 19, Issue 1, pp. 73-79, (2017) (PubMed).

Zawada, Mrak, Biedermann, Palmer, Gentleman, Aboud, Griffin et al.: "Loss of angiotensin II receptor expression in dopamine neurons in Parkinson's disease correlates with pathological progression and is accompanied by increases in Nox4- and 8-OH guanosine-related ..." in: Acta neuropathologica communications, Vol. 3, pp. 9, (2015) (PubMed).

Ozkosem, Feinstein, Fisher, OFlaherty: "Advancing age increases sperm chromatin damage and impairs fertility in peroxiredoxin 6 null mice." in: Redox biology, Vol. 5, pp. 15-23, (2015) (PubMed).

Angeloni, Malaguti, Rizzo, Barbalace, Fabbri, Hrelia: "Neuroprotective effect of sulforaphane against methylglyoxal cytotoxicity." in: Chemical research in toxicology, Vol. 28, Issue 6, pp. 1234-45, (2015) (PubMed).

Aboud, Mrak, Boop, Griffin: "Epilepsy: neuroinflammation, neurodegeneration, and APOE genotype." in: Acta neuropathologica communications, Vol. 1, pp. 41, (2013) (PubMed).

Brennan, Haukedal, Earle, Keddie, Harris: "Disruption of redox homeostasis leads to oxidative DNA damage in spermatocytes of Wolbachia-infected Drosophila simulans." in: Insect molecular biology, Vol. 21, Issue 5, pp. 510-20, (2012) (PubMed).

Target Details

Target: DNA Damage

Target Type: Chemical

Background: DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA due to endogenous cellular processes and they are oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS, occurs. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS causes a variety of issues including DNA damage(1). 8-hydroxyguanine, 8-hydroxy-2'-deoxyguanonsine and 8- hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2'-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring (3, 4). And finally, 8-hydroxy-guanosine, like 8-hydroxy-2'-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has specifically been tested in the development of diabetes, hypertension and strokes (5, 6, and 7).