TLR4 antibody (AA 420-435)

Catalog No. ABIN361724

Product Details anti-TLR4 Antibody

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Binding Specificity: AA 420-435

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Detects ~75-80 kDa when tested against partial recombinant mouse TLR4 (extra-cellular portion plus His-tag).

Cross-Reactivity: Human, Mouse

Purification: Protein A Purified

Immunogen: Developed against a synthetic peptide corresponding to amino acids 420-435 of human TLR4

Application Details

Application Notes:

• WB (1:500)
• IHC (1:50)
• optimal dilutions for assays should be determined by the user.

Comment:

2 μg/ml of ABIN361724 was sufficient for detection of TLR4 in 100 ng of partial recombinant mouse TLR4 protein by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-TLR4 antibody (ABIN361724)

Vercellotti, Dalmasso, Schaid, Nguyen, Chen, Ericson, Abdulla, Killeen, Lindorfer, Taylor, Belcher: "Critical role of C5a in sickle cell disease." in: American journal of hematology, Vol. 94, Issue 3, pp. 327-337, (2019) (PubMed).

Nakano, Furube, Morita, Wanaka, Nakashima, Miyata: "Astrocytic TLR4 expression and LPS-induced nuclear translocation of STAT3 in the sensory circumventricular organs of adult mouse brain." in: Journal of neuroimmunology, Vol. 278, pp. 144-58, (2015) (PubMed).

Takano, Ohkusa, Otani, Min, Kadoyama, Minami, Sano, Matsuyama: "Lipid A-activated inducible nitric oxide synthase expression via nuclear factor-κB in mouse choroid plexus cells." in: Immunology letters, Vol. 167, Issue 2, pp. 57-62, (2015) (PubMed).

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4 (TLR4 Products)

Synonyms: ARMD10 antibody, CD284 antibody, TLR-4 antibody, TOLL antibody, Lps antibody, Ly87 antibody, Ran/M1 antibody, Rasl2-8 antibody, toll like receptor 4 antibody, toll-like receptor 4 antibody, TLR4 antibody, Tlr4 antibody

Background: The Toll-like receptor (TLR) family in mammal comprises a family of trans-membrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and 1L-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TLR family is a phylo-genetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). Amount this family of receptors, TLR2 and TLR4 have been most studied. These studies have suggested that TLR2 and TLR4 may serve as potential main mediators of LPS signaling (4,5). The mouse TLR4 cDNA codes for a protein consisting of 839 amino acids, with an approximate molecular weight of 90 kDa (6).

Gene ID: 7099

NCBI Accession: NP_612564

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins