IFNAR1 antibody (pSer535, pSer539)

Catalog No. ABIN361505

Product Details anti-IFNAR1 Antibody

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Binding Specificity: pSer535, pSer539

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for IFNAR1 protein phosphorylated at Ser535,539.

Cross-Reactivity: Human

Predicted Reactivity: bovine, canine, mouse, non-human primate, rat, sheep

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-IFNAR1 antibody (ABIN361505)

Bhattacharya, HuangFu, Liu, Veeranki, Baker, Koumenis, Diehl, Fuchs: "Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2318-25, (2010) (PubMed).

Kumar, Krolewski, Fuchs: "Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 46614-20, (2004) (PubMed).

Target Details for IFNAR1

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Alternative Name: IFNAR1 (IFNAR1 Products)

Synonyms: ifnar1 antibody, IFNAR1 antibody, CD118 antibody, Ifar antibody, Ifnar antibody, Ifrc antibody, Infar antibody, AVP antibody, IFN-alpha-REC antibody, IFNAR antibody, IFNBR antibody, IFRC antibody, IFN-R-1 antibody, interferon alpha and beta receptor subunit 1 antibody, interferon-alpha/beta receptor alpha chain-like antibody, interferon (alpha and beta) receptor 1 antibody, interferon alpha/beta receptor 1 antibody, IFNAR1 antibody, ifnar1 antibody, Ifnar1 antibody, LOC609830 antibody

Background: Interferons are widely used therapeutic agents because of their anti tumor and antiviral effects and because of their modulatory effects on the immune system (Biron, 2001, Kirkwood, 2002). These cytokines produce their effects by binding to the Type 1 Interferon-a Receptor (IFNAR1). Down regulation of this receptor plays a key role in determining the magnitude and duration of cytokine signaling. This down regulation is thought to be influenced by phosphorylation of Serine 535 and 539 in the IFNAR1 (Kumar et al., 2003). Anti-Phospho Ser535,539 IFNAR1 Western blot of immunoprecipitates from HEK 293 cells transfected with 1. Mock, 2. IFNAR1 WT, and 3. IFNAR1 S535A and S539A mutants showing specific immunolabeling of the ~110k to ~130k IFNAR1 WT. The immunolabeling is absent in IFNAR1 Ser535 and Ser539 mutants (Control). The immunolabeling is blocked by the phosphopeptide (Phos) used as the antigen but not by the corresponding dephosphopeptide (Dephos).

Molecular Weight: 110-130 kDa

Gene ID: 3654

UniProt: P17181

Pathways: JAK-STAT Signaling, Hepatitis C