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SYT1 antibody (pSer309)

Name: SYT1 antibody (pSer309)
Brand: antibodies-online
SKU: ABIN361497
Price: 594 USD
Availability: InStock

SYT1 Reactivity: Rat WB, IHC Host: Rabbit Polyclonal unconjugated

Western blots of rat cortex lysate showing specific immunolabeling of the ~60k - ~62k synaptotagmin phosphorylated at Ser309 (Control). (SYT1 antibody (pSer309))

Western blot of rat cortical lysate showing specific immunolabeling of the ~62 kDa synaptotagmin phosphorylated at Thr202 in the first lane (-). (SYT1 antibody (pSer309))

Immunostaining of 14 DIV rat cortical neurons showing synaptotagmin when phosphorylated at Thr202 in red and PSD95 in green. (SYT1 antibody (pSer309))

ISt

3 images

(5 references)

Catalog No. ABIN361497

Datasheet as PDF

Target See all SYT1 Antibodies

SYT1 (Synaptotagmin I (SYT1))
Binding Specificity
15

12

8

8

5

5

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer309
Reactivity
78

67

48

11

7

6

6

4

4

4

4

2

2

2

1

1

1

1
Rat
Host
87

12

6

1

1

1
Rabbit
Compatible Secondaries
Clonality
96

12
Polyclonal
Conjugate
63

8

6

5

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This SYT1 antibody is un-conjugated
Application
63

38

34

16

13

13

13

11

7

6

4

2

2

1
Western Blotting (WB), Immunohistochemistry (IHC)

Specificity

Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309.

Cross-Reactivity

Rat (Rattus)

Predicted Reactivity

bovine, canine, chicken, human, mouse, non-human primate, zebra fish

Purification

Antigen Affinity Purified from Pooled Serum

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH

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SYT1 Reactivity: Human IHC, ELISA Host: Rabbit Polyclonal unconjugated

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SYT1 Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Synaptotagmin I (SYT1) (AA 349-377), (C-Term) antibody
SYT1 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB33649 unconjugated

Application Notes

Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.

Restrictions

For Research Use only
Format

Liquid

Buffer

100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage

-20 °C
Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Poskanzer, Marek, Sweeney, Davis: "Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo." in: Nature, Vol. 426, Issue 6966, pp. 559-63, (2003) (PubMed).

Wang, Lu, Bai, Chang, Martin, Chapman, Jackson: "Different domains of synaptotagmin control the choice between kiss-and-run and full fusion." in: Nature, Vol. 424, Issue 6951, pp. 943-7, (2003) (PubMed).

Fernández-Chacón, Königstorfer, Gerber, García, Matos, Stevens, Brose, Rizo, Rosenmund, Südhof: "Synaptotagmin I functions as a calcium regulator of release probability." in: Nature, Vol. 410, Issue 6824, pp. 41-9, (2001) (PubMed).

Hilfiker, Pieribone, Nordstedt, Greengard, Czernik: "Regulation of synaptotagmin I phosphorylation by multiple protein kinases." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 921-32, (1999) (PubMed).
Target

SYT1 (Synaptotagmin I (SYT1))

Alternative Name

SYT1 (SYT1 Products)

Molecular Weight

60/62 kDa

Gene ID

25716

UniProt

P21707

Pathways

Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport