SYN1 antibody (pSer62, pSer67)

Catalog No. ABIN361494

Product Details anti-SYN1 Antibody

Target: SYN1 (Synapsin I (SYN1))

Binding Specificity: pSer62, pSer67

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for ~78k synapsin I doublet phosphorylated at Ser62,67. Immunolabeling of the synapsin I band is blocked by preadsorption with the phospho-peptide used as antigen but not be the corresponding dephospho-peptide..

Cross-Reactivity: Rat (Rattus)

Predicted Reactivity: bovine, mouse

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser62/67 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-SYN1 antibody (ABIN361494)

Chanaday, Vilcaes, de Paul, Torres, Degano, Roth: "Glutamate release machinery is altered in the frontal cortex of rats with experimental autoimmune encephalomyelitis." in: Molecular neurobiology, Vol. 51, Issue 3, pp. 1353-67, (2015) (PubMed).

Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Tallent, Varghis, Skorobogatko, Hernandez-Cuebas, Whelan, Vocadlo, Vosseller: "In vivo modulation of O-GlcNAc levels regulates hippocampal synaptic plasticity through interplay with phosphorylation." in: The Journal of biological chemistry, Vol. 284, Issue 1, pp. 174-81, (2008) (PubMed).

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: SYN1 (SYN1 Products)

Synonyms: SYN1a antibody, SYN1b antibody, SYNI antibody, Syn-1 antibody, SYN I antibody, si:dkey-90n12.3 antibody, synapsin I antibody, synapsin I L homeolog antibody, SYN1 antibody, Syn1 antibody, syn1.L antibody, syn1 antibody

Background: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of Synapsin I that are phosphorylated by MAP kinase (Czernik et al., 1987, Jovanovic et al., 1996). Anti-Phospho-Ser62,67 Synapsin Western blot of rat cortex lysate showing specific labeling of the ~78k synapsin protein phosphorylated at Ser 62,67 (Control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Molecular Weight: '78 kDa

Gene ID: 281510

UniProt: P17599