SYN1 antibody (pSer549)

Catalog No. ABIN361492

Product Details anti-SYN1 Antibody

Target: SYN1 (Synapsin I (SYN1))

Binding Specificity: pSer549

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Specific for ~78k synapsin I doublet phosphorylated at Ser549. Immunolabeling of the synapsin I band is blocked by (-phosphatase treatment.

Cross-Reactivity: Rat (Rattus)

Predicted Reactivity: bovine, canine, human, mouse, non-human primate

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser549 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-SYN1 antibody (ABIN361492)

Dagyte, Luiten, De Jager, Gabriel, Mocaër, Den Boer, Van der Zee: "Chronic stress and antidepressant agomelatine induce region-specific changes in synapsin I expression in the rat brain." in: Journal of neuroscience research, Vol. 89, Issue 10, pp. 1646-57, (2011) (PubMed).

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: SYN1 (SYN1 Products)

Synonyms: SYN1a antibody, SYN1b antibody, SYNI antibody, Syn-1 antibody, SYN I antibody, si:dkey-90n12.3 antibody, synapsin I antibody, synapsin I L homeolog antibody, SYN1 antibody, Syn1 antibody, syn1.L antibody, syn1 antibody

Background: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002, Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001, Kao et al., 2002). Ser 549 along with Ser 62 and Ser 67 are the sites of synapsin I that are phosphorylated by MAP kinase (Jovanovic et al., 1996). Phosphorylation and subsequent dephosphorylation of this site is thought to play a key role in synaptic vesicle trafficking. Anti-Phospho-Ser549 Synapsin Western blot of rat cortex lysate showing specific immunolabeling of the ~78k synapsin I phosphorylated at Ser549 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho Ser549 synapsin I antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molecular Weight: '78 kDa

Gene ID: 281510

UniProt: P17599