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RPH3A antibody (pSer234)

RPH3A Reactivity: Rat WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN361489
  • Target See all RPH3A Antibodies
    RPH3A (Rabphilin 3A (RPH3A))
    Binding Specificity
    • 12
    • 10
    • 8
    • 6
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer234
    Reactivity
    • 42
    • 28
    • 25
    • 7
    • 7
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 47
    Rabbit
    Clonality
    • 47
    Polyclonal
    Conjugate
    • 30
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RPH3A antibody is un-conjugated
    Application
    • 38
    • 20
    • 13
    • 9
    • 3
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Specificity
    Specific for the ~82k rabphilin protein phosphorylated at Ser234. The immunolabeling of the rabphilin band was completely blocked by the phosphopeptide used as antigen while the dephosphopeptide had no effect on the immunolabeling
    Cross-Reactivity
    Rat (Rattus)
    Predicted Reactivity
    mouse
    Purification
    Antigen Affinity Purified from Pooled Serum
    Immunogen
    Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 conjugated to KLH
  • Application Notes
    Recommended Dilution: WB: 1:1000 IHC: 1:400 Quality Control: Western blots performed on each lot.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.
    Storage
    -20 °C
  • Stephens, Powell, Taraska, Harris: "Imaging the rapid yet transient accumulation of regulatory lipids, lipid kinases, and protein kinases during membrane fusion, at sites of exocytosis of MMP-9 in MCF-7 cells." in: Lipids in health and disease, Vol. 19, Issue 1, pp. 195, (2020) (PubMed).

  • Target
    RPH3A (Rabphilin 3A (RPH3A))
    Alternative Name
    RPH3A (RPH3A Products)
    Synonyms
    2900002P20Rik antibody, AU022689 antibody, AW108370 antibody, CG11556 antibody, Dmel\\CG11556 antibody, exophilin-1 antibody, fc61h10 antibody, rph3a antibody, wu:fc61h10 antibody, rabphilin 3A antibody, Rabphilin antibody, rabphilin-3a, putative antibody, rabphilin-3A antibody, rabphilin 3A S homeolog antibody, rabphilin 3A homolog (mouse), a antibody, RPH3A antibody, Rph3a antibody, Rph antibody, Smp_145120 antibody, Tsp_00335 antibody, rph3a.S antibody, rph3aa antibody
    Background
    Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994,Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington’s disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995,Foletti and Scheller, 2001). Anti-Phospho-Ser234 Rabphilin 3A Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.
    Molecular Weight
    '82 kDa
    Gene ID
    171039
    UniProt
    P47709
    Pathways
    Synaptic Vesicle Exocytosis
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