GAP43 antibody (pSer41)

Catalog No. ABIN361475

Product Details anti-GAP43 Antibody

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Binding Specificity: pSer41

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAP43 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~50k Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, that may be a Gap-43 aggregate or oligomer. Immunolableing is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is completely eliminated by treatment with (-Ptase.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, finch, human, non-human primates, Xenopus, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-GAP43 antibody (ABIN361475)

Yi, Kim, Park, Yu, Kim: "The effect of systemic injection of cyclosporin A on the phosphorylation of the PKC substrates MARCKS and GAP43 in the rat hippocampus." in: Neuroscience letters, Vol. 497, Issue 1, pp. 17-21, (2011) (PubMed).

Target Details for GAP43

Target: GAP43 (Growth Associated Protein 43 (GAP43))

Alternative Name: GAP43 (GAP43 Products)

Background: Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002). Anti-Phospho Ser41 Gap-43 Western blot of rat cortex lysate showing specific immunolabeling of the ~50k Gap-43 protein phosphorylated at Ser41 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the GAP-43 Ser41 antibody. The immunolabeling of GAP-43 is completely eliminated by treatment with (-Ptase.

Molecular Weight: '50 kDa

Gene ID: 29423

UniProt: P07936