GABBR2 antibody (pSer783)

Catalog No. ABIN361471

Product Details anti-GABBR2 Antibody

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Binding Specificity: pSer783

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABBR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Specific for ~102k GABAB R2 phosphorylated at Ser783. Immunolabeling of the GABAB R2 band is completely blocked by (-phosphatase treatment.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, human, non-human primates, Xenopus

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser783 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IF: 1:500 (Kuramoto et al., 2007) Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-GABBR2 antibody (ABIN361471)

Marron Fernandez de Velasco, Hearing, Xia, Victoria, Luján, Wickman: "Sex differences in GABABR-GIRK signaling in layer 5/6 pyramidal neurons of the mouse prelimbic cortex." in: Neuropharmacology, Vol. 95, pp. 353-60, (2015) (PubMed).

Target Details for GABBR2

Target: GABBR2 (gamma-aminobutyric Acid (GABA) B Receptor, 2 (GABBR2))

Alternative Name: GABBR2 (GABBR2 Products)

Synonyms: Gpr51 antibody, GABABR2 antibody, GPR51 antibody, GPRC3B antibody, HG20 antibody, HRIHFB2099 antibody, Gb2 antibody, Gm425 antibody, GABA(B)R2 antibody, si:dkey-190l1.2 antibody, gamma-aminobutyric acid type B receptor subunit 2 antibody, gamma-aminobutyric acid (GABA) B receptor, 2 antibody, gamma-aminobutyric acid (GABA) B receptor, 2 S homeolog antibody, GABBR2 antibody, LOC100448699 antibody, gabbr2 antibody, Gabbr2 antibody, gabbr2.S antibody

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors and phosphorylates S783 in the cytoplasmic tail of the R2 subunit and that S783 plays a critical role in enhancing neuronal survival after ischemia as phosphorylation of S783 is evident in many brain regions and is increased dramatically after ischemic injury to the brain (Kuramoto et al., 2007). Anti-Phospho-Ser783 GABAB R2 Western blot of rat synaptic membrane showing specific immunolabeling of the ~102 k GABAB R2 protein phosphorylated at Ser783 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser783 GABAB antibody. The immunolabeling is completely eliminated by treatment with (-Ptase.

Molecular Weight: '102 kDa

Gene ID: 83633

UniProt: O88871

Pathways: cAMP Metabolic Process