GABRG2 antibody (pSer327)

Catalog No. ABIN361470

Product Details anti-GABRG2 Antibody

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Binding Specificity: pSer327

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRG2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for ~45k GABAA receptor gamma2 subunit phosphorylated at Ser327. Immunolabeling of the GABAA band is completely blocked by lambda phosphatase treatment.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, human, non-human primate

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser327 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-GABRG2 antibody (ABIN361470)

Bohnsack, Carlson, Morrow: "Differential regulation of synaptic and extrasynaptic α4 GABA(A) receptor populations by protein kinase A and protein kinase C in cultured cortical neurons." in: Neuropharmacology, Vol. 105, pp. 124-32, (2016) (PubMed).

Target Details for GABRG2

Target: GABRG2 (gamma-aminobutyric Acid (GABA) A Receptor, gamma 2 (GABRG2))

Alternative Name: GABRG2 (GABRG2 Products)

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and sub-stance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha and beta subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma subunit is required for benzodiazepine modulation. It has recently been suggested that PKC regulates the sensitivity of GABAA alpha1beta2gamma2 receptors to ethanol and benzodiazepines through phosphorylation of serine 327 in the large intracellular loop of gamma2 (Qi et al., 2007)

Molecular Weight: '45 kDa

Gene ID: 29709

UniProt: P18508