EPH Receptor B2 antibody (pTyr298)

Catalog No. ABIN361466

Product Details anti-EPH Receptor B2 Antibody

Target: EPH Receptor B2 (EPHB2)

Binding Specificity: pTyr298

Reactivity: Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~46k EphrinB protein phosphorylated at Tyr298. Immunolabeling of the EphrinB band is blocked by (-phosphatase treatment.

Cross-Reactivity: Human, Rat (Rattus)

Predicted Reactivity: bovine, canine, chicken, human, mouse, non-human primate, Xenopus, zebra fish

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr298 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2 (EPHB2 Products)

Background: EphrinB proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development (Flanagan and Vancerhaeghen, 1998, Dufour et al., 2003, Oike et al., 2002). EphrinB molecules expressed at the membrane surface bind to the EphB family receptors on target cells during cell-to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing EphrinB on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of EphrinB is thought to be a critical component of this reverse signaling event (Palmer et al., 2002). Recent work suggests that phosphorylation of a specific EphrinB residue (Tyr298) plays a key role in EphrinB signaling (Kalo, et al., 2001). Anti-Phospho-Tyr298 EphrinB Western blot of rat testes lysate showing specific immunolabeling of the ~46k EphrinB phosphorylated at Tyr298 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: (-Ptase). The blot is identical to the control except that it was incubated in (-Ptase (1200 units for 30 min) before being exposed to the Anti-Tyr298 EphrinB. The immunolabeling of the EphrinB band is completely eliminated by treatment with (-Ptase.

Molecular Weight: '46 kDa

Gene ID: 396513

UniProt: P28693

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins