GABRA6 antibody (Cytoplasmic Loop)

Catalog No. ABIN361444

Product Details anti-GABRA6 Antibody

Target: GABRA6 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6))

Binding Specificity: Cytoplasmic Loop

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GABRA6 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Specific for the ~57k a6-subunit of the GABAA receptor in Western blots. Labeling is absent in a6-subunit knockout animals.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Purification: Antigen Affinity Purified

Immunogen: Fusion protein from the cytoplasmic loop of the alpha 6 subunit

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for GABRA6

Target: GABRA6 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 6 (GABRA6))

Alternative Name: GABRA6 (GABRA6 Products)

Synonyms: LOC100224835 antibody, GABRA6 antibody, Gabra-6 antibody, alpha6 antibody, si:dkey-217k23.1 antibody, zgc:73148 antibody, gamma-aminobutyric acid type A receptor alpha 6 subunit antibody, gamma-aminobutyric acid type A receptor alpha6 subunit antibody, gamma-aminobutyric acid (GABA) A receptor, subunit alpha 6 antibody, gamma-aminobutyric acid (GABA) A receptor, alpha 6a antibody, Gabra6 antibody, GABRA6 antibody, gabra6a antibody

Background: Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six (s, four (s and four (s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for (- and (-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a (-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different (-subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, P ltl et al., 2003). Anti-GABAA Receptor, a6-Subunit Western blot of mouse forebrain lysates from Wild Type (Control) and 6-knockout (6-K/O) animals showing specific immunolabeling of the ~57k a6-subunit of the GABAA-R. The labeling was absent from a lysate prepared from 6-knockout animals.

Molecular Weight: '57 kDa

Gene ID: 29708

UniProt: P30191

Pathways: Synaptic Membrane