NEFH antibody

Catalog No. ABIN361351

Product Details anti-NEFH Antibody

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Reactivity: Cow

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: Specific for the ~200k Neurofilament H protein.

Cross-Reactivity: Chicken, Mammalian

Purification: Total IgY fraction

Immunogen: Purified bovine NF-H

Application Details

Application Notes: Recommended Dilution: WB: 1:50,000 IF: 1:25,000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-NEFH antibody (ABIN361351)

Malara, Lutz, Incearap, Bauer, Cursano, Volbracht, Lerner, Pandey, Delling, Ioannidis, Arévalo, von Bernhardi, Schön, Bockmann, Dimou, Boeckers: "SHANK3 deficiency leads to myelin defects in the central and peripheral nervous system." in: Cellular and molecular life sciences : CMLS, Vol. 79, Issue 7, pp. 371, (2022) (PubMed).

Target Details for NEFH

Target: NEFH (Neurofilament, Heavy Polypeptide (NEFH))

Alternative Name: NEFH (NEFH Products)

Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDa, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) (2) and Alzheimer's disease (3). Anti-Neurofilament H Left: Western blot of rat cortex lysate showing specific immunolableing of the ~200k NF-H protein. Right: Immunofluorescence of rat cortical neurons and glia showing NF-H staining (red).

Molecular Weight: '200 kDa

Gene ID: 4744

UniProt: P12036