Actin antibody

Catalog No. ABIN361349

Product Details anti-Actin Antibody

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Actin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Specificity: Specific for the ~42 kDa actin protein in lysates from skeletal, cardiac, gizzard and aorta tissues. Reacts with all actin isoforms.

Cross-Reactivity: All Species

Purification: Ascites fluid

Immunogen: Preparation of chicken gizzard actin

Clone: C4

Isotype: IgG1

Application Details

Application Notes: Recommended Dilution: WB:1:5,000 IF: 1:500 IHC: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascites fluid + 0.01 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-Actin antibody (ABIN361349)

Wheaton, Aten, Queiroz, Sullivan, Oberdick, Hoyt, Obrietan: "Circadian expression and functional characterization of PEA-15 within the mouse suprachiasmatic nucleus." in: The European journal of neuroscience, Vol. 47, Issue 7, pp. 845-857, (2019) (PubMed).

Choi, Horning, Aten, Karelina, Alzate-Correa, Arthur, Hoyt, Obrietan: "Mitogen- and Stress-Activated Protein Kinase 1 Regulates Status Epilepticus-Evoked Cell Death in the Hippocampus." in: ASN neuro, Vol. 9, Issue 5, pp. 1759091417726607, (2018) (PubMed).

Barcus, OLeary, Brockman, Rugowski, Liu, Garcia, Yu, Keely, Eliceiri, Schuler: "Elevated collagen-I augments tumor progressive signals, intravasation and metastasis of prolactin-induced estrogen receptor alpha positive mammary tumor cells." in: Breast cancer research : BCR, Vol. 19, Issue 1, pp. 9, (2017) (PubMed).

Ramena, Yin, Yu, Walia, Elble: "CLCA2 Interactor EVA1 Is Required for Mammary Epithelial Cell Differentiation." in: PLoS ONE, Vol. 11, Issue 3, pp. e0147489, (2016) (PubMed).

Barcus, Holt, Keely, Eliceiri, Schuler: "Dense collagen-I matrices enhance pro-tumorigenic estrogen-prolactin crosstalk in MCF-7 and T47D breast cancer cells." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116891, (2016) (PubMed).

Target Details for Actin

Target: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Alternative Name: ACTA1 (ACTA1 Products)

Synonyms: ACTA antibody, ASMA antibody, CFTD antibody, CFTD1 antibody, CFTDM antibody, MPFD antibody, NEM1 antibody, NEM2 antibody, NEM3 antibody, acta antibody, asma antibody, cftd antibody, cftd1 antibody, cftdm antibody, mpfd antibody, nem1 antibody, AA959943 antibody, Acta-2 antibody, Acts antibody, Actsk-1 antibody, actc antibody, actc1 antibody, cfk antibody, hm:zeh0631 antibody, zeh0631 antibody, acta1 antibody, hm:zeh0293 antibody, zgc:112098 antibody, actin, alpha 1, skeletal muscle antibody, actin, alpha 1, skeletal muscle L homeolog antibody, actin, alpha 1b, skeletal muscle antibody, actin, muscle antibody, actin, alpha skeletal muscle antibody, actin, alpha 1a, skeletal muscle antibody, ACTA1 antibody, acta1.L antibody, acta1 antibody, Acta1 antibody, acta1b antibody, LOC100533357 antibody, LOC109479779 antibody, LOC109108280 antibody, acta1a antibody

Background: Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. Anti-Actin Western blot of rat hippocampal homogenate showing specific immunolabeling of the ~ 42k actin protein.

Molecular Weight: '42 kDa

Gene ID: 421534

UniProt: P68139

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development