MAP2 antibody

Catalog No. ABIN361345

Product Details anti-MAP2 Antibody

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Reactivity: Cow

Host: Chicken

Clonality: Polyclonal

Conjugate: This MAP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Specific for the ~ 280k MAP2 protein.

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Sensitivity: The antibody has been directly tested for reactivity in bovine, human, mouse and rat. It is expected that the antibody will react with other mammalian tissues.

Purification: Total IgY fraction

Immunogen: recombinant bovine MAP2 protein expressed in and purified from E. Coli

Application Details

Application Notes: Recommended Dilution: WB: 1:20,000 IF: 1:2,500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-MAP2 antibody (ABIN361345)

Woike, Wang, Tibbe, Hassani Nia, Failla, Kibæk, Overgård, Larsen, Fagerberg, Barsukov, Kreienkamp: "Mutations affecting the N-terminal domains of SHANK3 point to different pathomechanisms in neurodevelopmental disorders." in: Scientific reports, Vol. 12, Issue 1, pp. 902, (2022) (PubMed).

Hassani Nia, Woike, Bento, Niebling, Tibbe, Schulz, Hirnet, Skiba, Hönck, Veith, Günther, Scholz, Bierhals, Driemeyer, Bend, Failla, Lohr, Alai, Kreienkamp: "Structural deficits in key domains of Shank2 lead to alterations in postsynaptic nanoclusters and to a neurodevelopmental disorder in humans." in: Molecular psychiatry, (2022) (PubMed).

Hassani Nia, Woike, Martens, Klüssendorf, Hönck, Harder, Kreienkamp: "Targeting of δ-catenin to postsynaptic sites through interaction with the Shank3 N-terminus." in: Molecular autism, Vol. 11, Issue 1, pp. 85, (2021) (PubMed).

Hassani Nia, Woike, Kloth, Kortüm, Kreienkamp: "Truncating mutations in SHANK3 associated with global developmental delay interfere with nuclear β-catenin signaling." in: Journal of neurochemistry, (2020) (PubMed).

Ho, Dallalzadeh, Karathanasis, Keles, Vangala, Grogan, Poirazi, Martin: "GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons." in: Molecular and cellular neurosciences, Vol. 61, pp. 1-12, (2014) (PubMed).

Target Details for MAP2

Target: MAP2 (Microtubule-Associated Protein 2 (MAP2))

Alternative Name: MAP2 (MAP2 Products)

Synonyms: MAP2A antibody, MAP2B antibody, MAP2C antibody, MAP2R antibody, Mtap2 antibody, MAP-2 antibody, Mtap-2 antibody, xmap2 antibody, MGC53131 antibody, a730034c02 antibody, zgc:103474 antibody, zgc:113974 antibody, microtubule associated protein 2 antibody, microtubule-associated protein 2 antibody, microtubule associated protein 2 S homeolog antibody, MAP2 antibody, Map2 antibody, map2.S antibody, map2 antibody

Background: Microtubules are 25nm diameter protein rods found in most kinds of eukaryotic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein ( (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (Kindler & Gardner 1994). MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein ( (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. MAP2 has been recently shown to be the specific receptor for the neurosteroid pregnenolone (Fontaine-Lenore V. et al., 2006). Anti-MAP2 Left: Western blot of rat cortex lysate showing specific immunolabeling of the ~280k MAP2 protein. Right:Mixed neuron/glial cultures. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.

Molecular Weight: '280 kDa

Gene ID: 4133

UniProt: P11137