DLL1 antibody (Internal Region)

Catalog No. ABIN361190

Product Details anti-DLL1 Antibody

Target: DLL1 (delta-Like 1 (DLL1))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This DLL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Purpose: DLL1

Sequence: ATQRHLTVGE EWSQD

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-ATQRHLTVGEEWSQD, from the internal region of the protein sequence according to NP_005609.3

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 75 kDa band observed in Mouse Heart and Rat Pancreas lysates (calculated MW of 78.5 kDa according to Mouse NP_031891.2 and 77.4 kDa according to Rat NP_114452.1). An additional band of unknown identity was also consistently observed at

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: DLL1 (DLL1 Products)

Synonyms: X-delta-1 antibody, XDelta1 antibody, Xdelta-1 antibody, delta antibody, delta-1 antibody, delta1 antibody, x-delta antibody, DELTA1 antibody, DL1 antibody, Delta antibody, DLK antibody, DLK-1 antibody, Delta1 antibody, FA1 antibody, PREF1 antibody, Pref-1 antibody, ZOG antibody, pG2 antibody, DLL1 antibody, AW742678 antibody, DlkI antibody, Ly107 antibody, Peg9 antibody, SCP1 antibody, pref-1 antibody, delta-like 1 antibody, delta like canonical Notch ligand 1 antibody, delta like non-canonical Notch ligand 1 antibody, delta-like 1 L homeolog antibody, delta-like 1 (Drosophila) antibody, delta-like 1 homolog (Drosophila) antibody, dll1 antibody, DLL1 antibody, DLK1 antibody, Dll1 antibody, dll1.L antibody, Dlk1 antibody

Background: DLL1, delta-like 1 (Drosophila), DELTA1, DL1, Delta, OTTHUMP00000017690, delta (Drosophila)-like 1, delta-like 1, delta-like 1 protein

Gene ID: 28514, 13388, 84010

NCBI Accession: NP_005609

Pathways: Notch Signaling, Stem Cell Maintenance