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Granulin antibody (Internal Region)

Verified GRN Reactivity: Human ELISA, IF Host: Goat Polyclonal unconjugated
Catalog No. ABIN361185
  • Target See all Granulin (GRN) Antibodies
    Granulin (GRN)
    Binding Specificity
    • 15
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Internal Region
    Reactivity
    • 50
    • 9
    • 4
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 42
    • 9
    • 5
    • 1
    • 1
    Goat
    Clonality
    • 46
    • 12
    Polyclonal
    Conjugate
    • 35
    • 6
    • 5
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Granulin antibody is un-conjugated
    Application
    • 52
    • 17
    • 16
    • 13
    • 13
    • 10
    • 7
    • 7
    • 5
    • 3
    • 3
    ELISA, Immunofluorescence (IF)
    Purpose
    Granulin / GRN
    Sequence
    QSKCLSKENA TTD
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence QSKCLSKENATTD , from the internal region of the protein sequence according to NP_002078.1.
    Isotype
    IgG
    Top Product
    Discover our top product GRN Primary Antibody
  • Application Notes

    Western Blot: Preliminary experiments showed a band at approx 65 kDa in Human Bone Marrow and Testes lysates after 2 μg/mL antibody staining (calculated MW of 63.5 kDa according to NP_002078.1). Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:1000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the vesicles/cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    Granulin (GRN)
    Alternative Name
    GRN (GRN Products)
    Target Type
    Viral Protein
    Background
    GRN, granulin, GEP, GP88, PCDGF, PEPI, PGRN, PC cell-derived growth factor, acrogranin, granulin-epithelin, proepithelin, progranulin
    Gene ID
    2896
    NCBI Accession
    NP_002078
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