Klotho antibody

Catalog No. ABIN360924

Product Details anti-Klotho Antibody

Target: Klotho (KL)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Klotho antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody detects Mouse Klotho. Other species not tested.

Purification: Protein A/G Affinity Chromatography

Sterility: 0.2 μm filtered

Immunogen: Purified Mouse Recombinant protein of Klotho

Clone: 4F54

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry on frozen sections: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Buffer: 0.2 μm filtered solution in PBS, None

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Klotho

Target: Klotho (KL)

Alternative Name: Klotho (KL Products)

Background: Animal models of human aging which display the characteristic diseases associated with aging provide insight into the cause of such diseases. Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span. Klotho (Kl) protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane. kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on Cho cells. Human kl cDNA is expected to encode a protein of 1012 amino acids.Synonyms: EC 3.2.1.31, KL, Klotho

Gene ID: 16591

NCBI Accession: NP_038851

UniProt: O35082

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding