DGKA antibody (C-Term)
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- Target See all DGKA Antibodies
- DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This DGKA antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody reacts to DGKA.
- Purification
- Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human DGKA.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product DGKA Primary Antibody
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anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) antibody
DGKA Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) antibodyDGKA Reactivity: Human WB, IF Host: Mouse Polyclonal unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 305-531) antibodyDGKA Reactivity: Mouse WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) antibodyDGKA Reactivity: Human WB, IP Host: Mouse Monoclonal M6 unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-735) antibodyDGKA Reactivity: Human ELISA, IHC (p), PLA Host: Mouse Monoclonal 2B7 unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) antibodyDGKA Reactivity: Human, Dog, Monkey WB, IF Host: Mouse Monoclonal 8G5 unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 1-200) antibodyDGKA Reactivity: Human WB, IHC, ELISA Host: Rabbit Polyclonal unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 598-628), (C-Term) antibodyDGKA Reactivity: Human WB Host: Rabbit Polyclonal RB3872 unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) (Center) antibodyDGKA Reactivity: Human, Monkey WB, IHC, IF, IC Host: Rabbit Polyclonal unconjugated
anti-Diacylglycerol Kinase, alpha 80kDa (DGKA) (AA 312-557) antibodyDGKA Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
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- Application Notes
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ELISA: 1/1,000. Western Blot: 1/50 - 1/200. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- DGKA (Diacylglycerol Kinase, alpha 80kDa (DGKA))
- Alternative Name
- DAG Kinase alpha (DGKA Products)
- Background
- Upon cell stimulation, the kinase DGKA converts the second messenger diacylglycerol into phophatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. DGKA is stimulated by calcium and phosphatidylserine, and is phosphorylated by protein kinase C. Tissue expression is in lymphocytes and oligodengroglial cells. DGKA contains 2 zinc-dependent phorbol-ester and DAG binding domains, and 2 EF-hand calcium binding domains.Synonyms: DAGK, DAGK1, DGK-alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha
- Gene ID
- 1606, 9606
- UniProt
- P23743
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