PEPCK antibody (N-Term)

Catalog No. ABIN360567

Product Details anti-PEPCK Antibody

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PEPCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to PCK2.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PCK2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PEPCK

Target: PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))

Alternative Name: PCK2 (PEPCK Products)

Synonyms: PEPCK antibody, PEPCK-M antibody, PEPCK2 antibody, 1810010O14Rik antibody, 9130022B02Rik antibody, pck antibody, zgc:77867 antibody, id:ibd5157 antibody, pck2 antibody, pck2.S antibody, phosphoenolpyruvate carboxykinase 2, mitochondrial antibody, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody, phosphoenolpyruvate carboxykinase 2 (mitochondrial) L homeolog antibody, PCK2 antibody, Pck2 antibody, pck2 antibody, pck2.L antibody

Background: This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.Synonyms: EC=4.1.1.32, PCK-2, PEPCK-M, PEPCK2, Phosphoenolpyruvate carboxykinase, Phosphoenolpyruvate carboxylase

Gene ID: 5106, 5874

UniProt: Q16822

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis