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NEK1 antibody (C-Term)

NEK1 Reactivity: Human, Hamster WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN360536
  • Target See all NEK1 Antibodies
    NEK1 (NIMA-Related Kinase 1 (NEK1))
    Binding Specificity
    • 15
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    AA 1173-1202, C-Term
    Reactivity
    • 23
    • 22
    • 15
    • 1
    Human, Hamster
    Host
    • 38
    Rabbit
    Clonality
    • 38
    Polyclonal
    Conjugate
    • 11
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NEK1 antibody is un-conjugated
    Application
    • 21
    • 13
    • 13
    • 12
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody is specific to NEK1.
    Purification
    Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
    Immunogen
    KLH conjugated synthetic peptide between 1173~1202 amino acids from the C-terminal region of Human NEK1.
    Isotype
    Ig Fraction
    Top Product
    Discover our top product NEK1 Primary Antibody
  • Application Notes
    ELISA: 1/1,000. Western blotting: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.25 mg/mL
    Buffer
    PBS with 0.09 % (W/V) Sodium Azide as preservative
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • Target
    NEK1 (NIMA-Related Kinase 1 (NEK1))
    Alternative Name
    NEK1 (NEK1 Products)
    Synonyms
    D8Ertd790e antibody, kat antibody, NY-REN-55 antibody, SRPS2 antibody, SRPS2A antibody, ATNEK1 antibody, F20D21.32 antibody, F20D21_32 antibody, NIMA-RELATED KINASE1 antibody, NIMA-related serine/threonine kinase 1 antibody, NIMA (never in mitosis gene a)-related expressed kinase 1 antibody, NIMA related kinase 1 antibody, NIMA-related kinase 1 antibody, NIMA-related serine/threonine kinase 1 antibody, Nek1 antibody, NEK1 antibody
    Background
    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.Synonyms: NimA-related protein kinase 1, NimA-related protein kinase 10, Renal carcinoma antigen NY-REN-55, Serine/threonine-protein kinase Nek1
    Gene ID
    4750
    NCBI Accession
    NP_001186326
    UniProt
    Q96PY6
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