ELISA: 1/1,000. Western blot: 1/50-1/100. Immunohistochemistry: 1/10-1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet <--> AMP + diphosphate + L-methionyl-tRNAMet. The 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met). This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.Synonyms: Methionine-tRNA ligase 2, Methionyl-tRNA synthetase, MtMetRS, mitochondrial