Protein A Chromatography followed by peptide affinity purification.
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Ser182 of Human PUM2.
PUM2
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
7C7
unconjugated
Application Notes
ELISA: 1/1,000. Western blot 1/50approx. 1/100. Immunohistochemistry: 1/10approx. 1/50. Flow Cytometry: 1/10approx. 1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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Background
PUM2 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.Synonyms: KIAA0235, PUM-2, PUMH2, Pumilio homolog 2, Pumilio-2