Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CAMK1-like protein kinase.
CAMK1D
Reactivity: Human, Mouse, Rat
IF
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
Target
CAMK1D
(Calcium/calmodulin-Dependent Protein Kinase ID (CAMK1D))
CKLiK antibody, CaM-K1 antibody, CaMKID antibody, A630059D12Rik antibody, CaMKIdelta antibody, E030025C11Rik antibody, CAMK1D antibody, camk1d antibody, zgc:158713 antibody, RGD1560691 antibody, zgc:172284 antibody, cam-ki antibody, camk1d.L antibody, calcium/calmodulin dependent protein kinase ID antibody, calcium/calmodulin-dependent protein kinase ID antibody, calcium/calmodulin-dependent protein kinase 1Db antibody, calcium/calmodulin-dependent protein kinase 1Da antibody, calcium/calmodulin dependent protein kinase ID S homeolog antibody, CAMK1D antibody, Camk1d antibody, camk1db antibody, LOAG_08089 antibody, camk1da antibody, camk1d.S antibody
Background
CAMK1-like protein kinase belongs to a proposed calcium-triggered signaling cascade. This protein is expressed in polymorphonuclear leukocytes (PMNs) and may be part of the chemokine signal transduction pathway that regulates granulocyte function. CAMK1-like protein kinase may play a role in apoptosis of erythroleukemia cells. It activates MAP kinase MAPK3, and in vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1.Synonyms: CKLiK, CaM kinase I delta, CaM kinase ID, CaMKI-delta, Calcium/calmodulin-dependent protein kinase type 1D, CamKI-like protein kinase, Camk1D