Sclerostin antibody (N-Term)

Catalog No. ABIN358751

Product Details anti-Sclerostin Antibody

Target: Sclerostin (SOST)

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Sclerostin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to SOST.

Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SOST.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-Sclerostin antibody (ABIN358751)

Sato, Cregor, Delgado-Calle, Condon, Allen, Peacock, Plotkin, Bellido: "Protection From Glucocorticoid-Induced Osteoporosis by Anti-Catabolic Signaling in the Absence of Sost/Sclerostin." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 31, Issue 10, pp. 1791-1802, (2016) (PubMed).

Target Details for Sclerostin

Target: Sclerostin (SOST)

Alternative Name: Sclerostin / SOST (SOST Products)

Synonyms: LOC100313724 antibody, CDD antibody, VBCH antibody, 5430411E23Rik antibody, sclerostin antibody, LOC100313724 antibody, SOST antibody, Sost antibody

Background: Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.

Gene ID: 50964, 9606

UniProt: Q9BQB4