Caspase 6 antibody (pSer257)

Catalog No. ABIN358091

Product Details anti-Caspase 6 Antibody

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Binding Specificity: pSer257

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects Caspase-6 pSer257. Predicted to cross react with Mouse (100 % Antigen Homology).

Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S257 of human caspase 6.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Caspase 6

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Alternative Name: Caspase-6 (CASP6 Products)

Synonyms: xCaspase-6 antibody, mch2 antibody, caspase-6 antibody, zgc:112960 antibody, CASP6 antibody, casp6 antibody, Mch2 antibody, mCASP-6 antibody, MCH2 antibody, caspase 6 L homeolog antibody, caspase 6 antibody, caspase 6, apoptosis-related cysteine peptidase antibody, group XIIA secretory phospholipase A2 antibody, caspase-6 antibody, casp6.L antibody, casp6 antibody, CASP6 antibody, CpipJ_CPIJ017008 antibody, Casp6 antibody

Background: Caspase 6 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This protein could be processed by caspases 7, 8 and 10, and is thought to function as a downstream enzyme in the caspase activation cascade.Synonyms: Apoptotic protease Mch-2, CASP-6, CASP6, MCH-2, MCH2

Molecular Weight: 33310 Da

Gene ID: 839, 9606

UniProt: P55212

Pathways: Apoptosis, Caspase Cascade in Apoptosis