SRSF2 antibody (N-Term)

Catalog No. ABIN357967

Product Details anti-SRSF2 Antibody

Target: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SRSF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects SFRS2 (N-term).

Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SFRS2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/50-1/100. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for SRSF2

Target: SRSF2 (serine/arginine-Rich Splicing Factor 2 (SRSF2))

Alternative Name: SFRS2 / SC-35 (SRSF2 Products)

Synonyms: PR264 antibody, SC-35 antibody, SC35 antibody, SFRS2 antibody, SFRS2A antibody, SRp30b antibody, D11Wsu175e antibody, MRF-1 antibody, Pr264 antibody, Sfrs10 antibody, Sfrs2 antibody, pr264 antibody, sc-35 antibody, sc35 antibody, sfrs2 antibody, sfrs2a antibody, srp30b antibody, serine and arginine rich splicing factor 2 antibody, serine/arginine-rich splicing factor 2 antibody, serine/arginine-rich splicing factor 2 L homeolog antibody, SRSF2 antibody, Srsf2 antibody, srsf2.L antibody

Background: SFRS2 is necessary for the splicing of pre-mRNA. The protein is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. And it interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. It binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3' and can bind to beta-globin mRNA and commit it to the splicing pathway.Synonyms: Nuclear Speckle Marker, PR264, Splicing component 35 kDa, Splicing factor SC35, Splicing factor arginine/serine-rich 2

Molecular Weight: 25476 Da (splicing protein is 35 KD).

Gene ID: 6427, 9606

UniProt: Q01130