Ataxin 3 antibody (N-Term)

Catalog No. ABIN357621

Product Details anti-Ataxin 3 Antibody

Target: Ataxin 3 (ATXN3)

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ataxin 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific to Ataxin-3 (N-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human MJD.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Ataxin 3

Target: Ataxin 3 (ATXN3)

Alternative Name: Ataxin-3 (ATXN3 Products)

Synonyms: Mjd antibody, zgc:56323 antibody, ATXN3 antibody, at3 antibody, jos antibody, mjd antibody, atx3 antibody, mjd1 antibody, sca3 antibody, ataxin-3 antibody, MJD1 antibody, MGC83584 antibody, AT3 antibody, ATX3 antibody, JOS antibody, MJD antibody, SCA3 antibody, 2210008M02Rik antibody, AI463012 antibody, AI647473 antibody, Sca3 antibody, Rsca3 antibody, ataxin 3 antibody, ataxin 3 L homeolog antibody, ataxin-3 antibody, atxn3 antibody, ATXN3 antibody, atxn3.L antibody, MICPUN_104046 antibody, MICPUCDRAFT_46658 antibody, Atxn3 antibody

Background: Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.Synonyms: ATX3, ATXN3, MJD, MJD1, Machado-Joseph disease protein 1, SCA3, Spinocerebellar ataxia type 3 protein

Molecular Weight: 43450 Da

Gene ID: 4287, 9606

UniProt: P54252