USP14 antibody (N-Term)

Catalog No. ABIN357546

Product Details anti-USP14 Antibody

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific to USP14 (N-term). Predicted to cross react with Mouse (100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human USP14.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for USP14

Target: USP14 (Ubiquitin Specific Peptidase 14 (USP14))

Alternative Name: USP14 (USP14 Products)

Synonyms: TGT antibody, DDBDRAFT_0202821 antibody, DDBDRAFT_0237695 antibody, DDB_0202821 antibody, DDB_0237695 antibody, 2610005K12Rik antibody, 2610037B11Rik antibody, AW107924 antibody, C78769 antibody, ax antibody, zgc:55949 antibody, wu:fk63d09 antibody, MGC81945 antibody, tgt antibody, MGC69343 antibody, USP14 antibody, ubiquitin specific peptidase 14 antibody, peptidase C19 family protein antibody, ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) antibody, ubiquitin specific protease 14 antibody, ubiquitin specific peptidase 14 S homeolog antibody, universal stress protein antibody, Ubiquitin carboxyl-terminal hydrolase 14 antibody, USP14 antibody, usp14 antibody, Usp14 antibody, Bm1_28765 antibody, usp14.S antibody, RR_RS06480 antibody, usp-14 antibody

Background: Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.Synonyms: Deubiquitinating enzyme 14, TGT, Ubiquitin carboxyl-terminal hydrolase 14, Ubiquitin thioesterase 14, Ubiquitin-specific-processing protease 14

Molecular Weight: 55938 Da

Gene ID: 9097, 5874

UniProt: P54578