USP12 antibody (N-Term)
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- Target See all USP12 Antibodies
- USP12 (Ubiquitin Specific Peptidase 12 (USP12))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This USP12 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is specific to USP12 (N-term).
- Purification
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
- Immunogen
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human USP12.
- Isotype
- Ig Fraction
- Top Product
- Discover our top product USP12 Primary Antibody
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- Application Notes
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ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- USP12 (Ubiquitin Specific Peptidase 12 (USP12))
- Alternative Name
- USP12 (USP12 Products)
- Synonyms
- UBH1 antibody, USP12L1 antibody, usp12 antibody, USP12P1 antibody, sb:eu882 antibody, wu:fi09d12 antibody, Ubh1 antibody, ubh1 antibody, usp12l1 antibody, ubiquitin specific peptidase 12 antibody, ubiquitin specific peptidase 12a antibody, ubiquitin specific peptidase 12 L homeolog antibody, ubiquitin specific peptidase 12 S homeolog antibody, USP12 antibody, usp12 antibody, Usp12 antibody, usp12a antibody, usp12.L antibody, usp12.S antibody
- Background
- Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.Synonyms: Deubiquitinating enzyme 12, UBH1, USP12L1, Ubiquitin carboxyl-terminal hydrolase 12, Ubiquitin thioesterase 12, Ubiquitin-hydrolyzing enzyme 1, Ubiquitin-specific-processing protease 12
- Molecular Weight
- 41201 Da
- Gene ID
- 219333, 5874
- UniProt
- O75317
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