UBE2G1 antibody (N-Term)

Catalog No. ABIN357499

Product Details anti-UBE2G1 Antibody

Target: UBE2G1 (Ube2g1) (Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2G1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific to UBE2G1 (N-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human UBE2G1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for UBE2G1

Target: UBE2G1 (Ube2g1) (Ubiquitin-Conjugating Enzyme E2G 1 (Ube2g1))

Alternative Name: UBE2G1 (Ube2g1 Products)

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2G1 is a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 98-100 % sequence identity with the zebrafish, frog, rat and mouse counterparts, which indicates that this enzyme is highly conserved in eukaryotes.Synonyms: E217K, UBC7, UBE2G, Ubiquitin carrier protein G1, Ubiquitin-conjugating enzyme E2 G1, Ubiquitin-protein ligase G1

Molecular Weight: 19509 Da

Gene ID: 7326, 5874

UniProt: P62253