UBA1 antibody (C-Term)

Catalog No. ABIN357486

Product Details anti-UBA1 Antibody

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody is specific to UBE1/UBA1 (C-term). Predicted to cross react with Mouse and Rat (100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human UBE1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for UBA1

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Alternative Name: UBE1 / UBA1 (UBA1 Products)

Synonyms: A1S9 antibody, A1S9T antibody, A1ST antibody, AMCX1 antibody, GXP1 antibody, POC20 antibody, SMAX2 antibody, UBA1A antibody, UBE1 antibody, UBE1X antibody, DDBDRAFT_0190927 antibody, DDBDRAFT_0220497 antibody, DDB_0190927 antibody, DDB_0220497 antibody, Sbx antibody, Ube-1 antibody, Ube1x antibody, a1s9 antibody, a1s9t antibody, a1st antibody, amcx1 antibody, gxp1 antibody, poc20 antibody, smax2 antibody, uba1a antibody, ube1 antibody, ube1x antibody, uba1 antibody, uba1b antibody, ube antibody, zgc:66143 antibody, wu:fa01e08 antibody, wu:fb30f01 antibody, wu:fi21c11 antibody, wu:fj14g11 antibody, ubiquitin like modifier activating enzyme 1 antibody, ubiquitin activating enzyme E1 antibody, Ubiquitin-conjugating enzyme E1 antibody, ubiquitin-like modifier activating enzyme 1 antibody, ubiquitin-like modifier activating enzyme 1 S homeolog antibody, ubiquitin-like modifier activating enzyme 1 L homeolog antibody, UBA1 antibody, uae1 antibody, GL50803_4083 antibody, GL50803_10661 antibody, Uba1 antibody, uba1.S antibody, uba1.L antibody, uba1 antibody, ptr3 antibody

Background: UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5' UTR.Synonyms: A1S9T, Protein A1S9, Ubiquitin-activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1

Molecular Weight: 117848 Da

Gene ID: 7317, 5874

UniProt: P22314