TPX2 antibody (C-Term)

Catalog No. ABIN357426

Product Details anti-TPX2 Antibody

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects P100.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with mixture of 3 KLH conjugated synthetic peptides selected from C-terminal region with phenylalanine at position 624, C- terminal region with serine at position 729, and center region of human NRG4.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for TPX2

Target: TPX2 (TPX2, Microtubule-Associated, Homolog (Xenopus Laevis) (TPX2))

Alternative Name: TPX2 / DIL2 (TPX2 Products)

Background: The nuclear protein p100 is a proliferation-associated protein whose expression is restricted to cell cycle phases S, G2, and M. Exclusively expressed in proliferating cells from the transition G1/S until the end of cytokinesis. During mitosis it is strictly associated with the spindle pole and with the mitotic spindle, whereas during S and G2, it is diffusely distributed throughout the nucleus. The full-length cDNA encodes a 747-amino acid protein with a putative ATP/GTP-binding site motif. RT-PCR analysis demonstrated strong expression of in lung carcinoma cell lines but not in normal lung tissues. Expression was also found in adult placenta, skeletal muscle, thymus, testis, and small intestine and in fetal brain, liver, and kidney. P100 is also correlated to cancer prognosis.Synonyms: C20orf1, C20orf2, DIL-2, Differentially expressed in cancerous and non-cancerous lung cells 2, HCA519, Hepatocellular carcinoma-associated antigen 519, Restricted expression proliferation-associated protein 100, Targeting protein for Xklp2

Molecular Weight: 85653 Da

Gene ID: 22974, 9606

UniProt: Q9ULW0