PUMA antibody (BH3 Domain, C-Term)
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- Target See all PUMA (BBC3) Antibodies
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
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Binding Specificity
- BH3 Domain, C-Term
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Reactivity
- Human, Mouse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PUMA antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is specific to Human and Mouse PUMA (BH3 Domain). Other species not tested.
- Purification
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Immunogen
- Synthetic peptide selected from the C-terminal region of Human PUMA
- Isotype
- Ig Fraction
- Top Product
- Discover our top product BBC3 Primary Antibody
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anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody
BBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 131-180) antibodyBBC3 Reactivity: Human, Rat, Pig WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 Reactivity: Human WB, FACS, IHC (p), IF Host: Rabbit Polyclonal RB01353 unconjugated
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibodyBBC3 Reactivity: Human WB, ELISA, IHC, FACS, IF Host: Rabbit Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibodyBBC3 Reactivity: Human WB, IHC Host: Rabbit Polyclonal unconjugated
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PE)BBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal PE
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody (PerCP)BBC3 Reactivity: Human WB, ICC, IF Host: Rabbit Polyclonal PerCP
anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody (Biotin)BBC3 Reactivity: Human WB, IHC Host: Rabbit Polyclonal Biotin
anti-BCL2 Binding Component 3 (BBC3) (AA 109-137) antibodyBBC3 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal RB33714 unconjugated
anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibodyBBC3 Reactivity: Human, Mouse WB, IHC, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated
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- Application Notes
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ELISA: 1/1,000. Western blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS containing 0.09 % (W/V) Sodium Azide as preservative
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Target
- PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
- Alternative Name
- PUMA (BBC3 Products)
- Synonyms
- BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
- Background
- PUMA is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-a and PUMA-b. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
- Molecular Weight
- 20532 Da
- Gene ID
- 27113, 9606
- UniProt
- Q9BXH1
- Pathways
- p53 Signaling, Positive Regulation of Endopeptidase Activity
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