CD175s antibody
Reactivity: Human
FACS, IHC (fro), IF, IHC (p)
Host: Mouse
Monoclonal
STN218
unconjugated
(1 reference)-
- Target
- CD175s
- Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Un-conjugated
- Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Characteristics
- Synonyms: Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn, NeuAc a GalNAc
- Purification
- Purified
- Immunogen
- Ovine submaxillary mucin (OSM)Remarks: Molecular weight of antigen: Several HM weight glycoproteins. Epitope: NeuAc alphaGalNacOSer/Thr.
- Clone
- STN218
- Isotype
- IgG1
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- Application Notes
-
Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Immunofluorescence. Flow Cytometry. Recommended Positive Control: Human gastrointestinal tumor, prostate, ovary ca
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
-
- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
-
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: One year from despatch. - Expiry Date
- 12 months
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-
: "COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer." in: Molecular cancer, Vol. 14, pp. 109, (2015) (PubMed).
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: "COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer." in: Molecular cancer, Vol. 14, pp. 109, (2015) (PubMed).
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- Target
- CD175s
- Background
- Sialosyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.Synonyms: NeuAc a GalNAc, Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn
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