CD175s antibody (FITC)
Reactivity: Human
FACS, IHC (fro), IF
Host: Mouse
Monoclonal
STN218
FITC
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- Target
- CD175s
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- FITC
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)
- Characteristics
- Synonyms: Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn, NeuAc a GalNAc
- Immunogen
- Ovine submaxillary mucin (OSM). Remarks: Molecular weight of antigen: Several HM weight glycoproteins. Epitope: NeuAc alphaGalNacOSer/Thr.
- Clone
- STN218
- Isotype
- IgG1
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- Application Notes
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Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears)For IHC dilute concentared antibody at 1/50-1/100, use streptavidinapprox. biotin system orpolymer system, incubate 30 minutes at room temperature. Immunofluorescence: 10-20 μg/mL (1/10-1/20), incubate for 2 hours in the dark at RT or itcan also be incubated overnight at 4 °C. Flow Cytometry: 0.2-1.0 μg/0.1 mL(1/200-1/1,000). Recommended Positive Control: Human gastrointestinal tumor, prostate, ovary ca.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- PBS, pH 7.4 containing 1 % BSA as stabilizer and 0.05 % Sodium Azide as preservative.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Shelf life: One year from despatch. - Expiry Date
- 12 months
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- Target
- CD175s
- Background
- Sialosyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialosyl Tn has limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections.Synonyms: NeuAc a GalNAc, Sialosyl Tn, Sialosyl-Tn, Sialyl Tn, sTn
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